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KMID : 0613820040140020368
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Journal of Life Science
2004 Volume.14 No. 2 p.368 ~ p.373
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Expression of Phosphinothricin Acetyltransferase Gene in Transgenic Rice Plants
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Lee Soo-In
Lee Sung-Ho
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Abstract
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We have obtained fertile transgenic rice plants resistant to the broad spectrum herbicide Basta® (active ingredient phosphinothricin, PPT) by PEG-mediated transformation procedure. The plasmid pCaMV35S:: Bar was used to deliver the bar gene into embryogenic suspension culture-derived protoplasts of rice (Oryza sativa L.). Transformed plants were regenerated and selected on medium containing 15 mg/l of phosphinothricin. Stable integration and expression of the bar gene in transgenic rice plants was confirmed by Southern and Northern blot analysis. Transgenic R1 plants were also confirmed by assays for phosphinothricin acetyltransferase (PAT) activity. The bar gene was expressed in the primary transgenic rice plants and in the next generation progeny, in which it showed a 3:1 Mendelian inheritance pattern. Transgenic R1 and R2 plants were resistant to the herbicide Basta® when sprayed at rates used in field practice.
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KEYWORD
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herbicide, Oryza sativa, protoplast, transformation
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